Publications and Patents
Complete list of publications found here
Protein Engineering
Cruz-Teran C.A., Bacon K., Rao B.M. (2020) Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae Using Inefficient Ribosomal Skipping. In: Zielonka S., Krah S. (eds) Genotype Phenotype Coupling. Methods in Molecular Biology, vol 2070. Humana, New York, NY
Bacon K., Burroughs M., Blain A., Menegatti S., Rao B.M., Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders, ACS Combinatorial Science 2019
Cruz-Teran C.A., Bacon K., McArthur N., Rao B.M., An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells, ACS Combinatorial Science 2018
Cruz-Teran C.A., Tiruthani K., Mischler A., Rao B.M. Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in Saccharomyces cerevisiae, ACS Synthetic Biology 2017
Canbolat, M.F., Gera, N., Tang, C., Monian, B., Rao, B.M., Pourdeyhimi, B., Khan, S.A., Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast, ACS Applied Materials & Interfaces 2013
Goli, K.K., Gera, N., Liu, X., Rao, B.M., Rojas, O.J., Genzer, J., Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fibers, ACS Applied Materials & Interfaces 2013
Gera, N., Hill, A.B., White, D.P., Carbonell, R.G., Rao, B.M., Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold, PLoS ONE 2012
Hussain, M., Lockney, D., Wang, R., Gera, N., Rao, B.M., Avidity-mediated virus separation using a hyperthermophilic affinity ligand, Biotechnology Progress 2012
Menegatti, S.,Hussain, M., Naik, A., Carbonell, R.G., Rao, B.M., mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands, Biotechnology and Bioengineering 2012
Hussain, M.*, Gera, N.*, Hill, A.B., Rao, B.M., Scaffold diversification enhances effectiveness of a super-library of hyperthermophilic proteins, ACS Synthetic Biology 2012
* These authors contributed equally
Gera, N., Hussain, M., Rao, B.M., Protein Selection using Yeast Cell Surface Display, Methods 2012
Gera, N., Hussain, M., Wright, R.C., Rao, B.M., Highly stable binding proteins derived from the hyperthermophilic Sso7d scaffold, Journal of Molecular Biology 2011 Jun;409(4):601-16
Cochran, J. R., Kim, Y.S., Lippow, S., Rao, B.M. and Wittrup, K. D. Improved mutants from directed evolution are biased to orthologous substitutions, Protein Engineering Design and Selection 2006 Jun; 19(6): 245-253
Rao, B.M., Driver, I., Lauffenburger, D. A., and Wittrup, K. D. High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth,Biochemistry 2005 Aug; 44(31): 10696-10701
Rao, B.M., Lauffenburger, D. A., and Wittrup, K. D. Engineering protein therapeutics within a systems-level computational context, Nature Biotechnology 2005 Feb; 23(2): 191-194
Rao, B.M., Driver, I., Lauffenburger, D. A., and Wittrup, K. D. IL-2 variants engineered for increased IL-2R-alpha affinity exhibit increased potency arising from a cell surface ligand reservoir effect, Molecular Pharmacology 2004 Oct; 66(4): 864-869
Rao, B.M., Girvin, A. T., Ciardelli, T., Lauffenburger, D. A., and Wittrup, K. D. Interleukin-2 mutants with increased alpha-subunit binding affinity, Protein Engineering 2003 Dec; 16(12): 1081 1087
Stem Cell Engineering
Tiruthani. K., Sarkar, P., Rao, B.M., Trophoblast differentiation of human embryonic stem cells, Biotechnology Journal. 2013 doi:10.1002/biot.201200203
Sarkar, P., Randall, S.M., Muddiman, D.C., Rao, B.M., Targeted proteomics of the secretory pathway reveals the secretome of mouse embryonic fibroblasts and human embryonic stem cells, Molecular & Cellular Proteomics 2012 ; Dec;11(12):1829-39
Sarkar, P., Collier, T.S., Randall, S.M., Muddiman, D.C., Rao, B.M., The subcellular proteome of undifferentiated human embryonic stem cells, Proteomics 2012 Feb 12(3):421-30
Sarkar, P., Rao, B.M., Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation,Embryonic Stem Cells – Differentiation and Pluripotent Alternatives 2011 Oct
Collier, T.S., Randall, S.M., Sarkar, P., Rao, B.M, Dean, R.A., Muddiman, D.C., Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression, Rapid Communications in Mass Spectrometry 2011 Sep;25(17):2524-32
Collier, T.S., Sarkar, P., Franck, W.L., Rao, B.M., Dean, R.A., Muddiman, DC., Direct comparison of stable isotope labeling by amino acids in cell culture and spectral counting for quantitative proteomics, Analytical Chemistry 2010 Oct;82(20):8696-702
Collier, T.S., Sarkar, P., Rao, B.M., Muddiman, D.C., Quantitative top-down proteomics of SILAC labeled human embryonic stem cells, Journal of the American Society for Mass Spectrometry 2010 Jun;21(6):879-89
Sarkar, P., Rao, B.M., Molecular aspects of cardiac differentiation in embryonic stem cells, Critical Reviews in Biomedical Engineering 2009;37(4-5):283-320
Varelas, X., Sakuma, R., Samavarchi-Tehrani, P., Peerani, R., Rao, B.M., Dembowy, J., Yaffe, M. B., Zandstra, P.W. and Wrana, J.L. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal, Nature Cell Biology 2008 Jul;10(7):837-48
Peerani, R.*, Rao, B.M.*, Bauwens, C., Yin, T., Wood, G., Nagy, A., Kumacheva, E., Zandstra, P. W. Niche-mediated control of human embryonic stem cell self-renewal and differentiation, EMBO Journal 2007 Nov; 26(22): 4744-4755
* These authors contributed equally
Rao, B.M. and Zandstra, P.W. Culture development for human embryonic stem cell propagation: molecular aspects and challenges, Current Opinion in Biotechnology 2005 Oct; 16(5): 568-576
Patents
Wittrup, K. D., Rao, B.M. and Lauffenburger, D. A. Mutant Interleukin-2 Polypeptides (US Application No. 10/894,833)