{"id":17,"date":"2017-02-05T14:39:59","date_gmt":"2017-02-05T19:39:59","guid":{"rendered":"https:\/\/cbe.ncsu.edu\/raogroup\/?page_id=17"},"modified":"2026-04-15T09:48:58","modified_gmt":"2026-04-15T13:48:58","slug":"publications-and-patents","status":"publish","type":"page","link":"https:\/\/cbe.ncsu.edu\/raogroup\/publications-and-patents\/","title":{"rendered":"Publications and Patents"},"content":{"rendered":"

Complete list of publications found here<\/span><\/a><\/p>\n

<\/h3>\n

Protein Engineering<\/h2>\n

Cruz-Teran C.A., Bacon K., Rao B.M. (2020) Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces cerevisiae<\/em>\u00a0Using Inefficient Ribosomal Skipping. In: Zielonka S., Krah S. (eds) Genotype Phenotype Coupling. Methods in Molecular Biology, vol 2070<\/a>. Humana, New York, NY<\/p>\n

Bacon K., Burroughs M., Blain A., Menegatti S., Rao B.M., Screening Yeast Display Libraries against Magnetized Yeast Cell Targets Enables Efficient Isolation of Membrane Protein Binders, ACS Combinatorial Science 2019<\/a><\/p>\n

Cruz-Teran C.A., Bacon K., McArthur N., Rao B.M., An Engineered Sso7d Variant Enables Efficient Magnetization of Yeast Cells, ACS Combinatorial Science 2018<\/a><\/p>\n

Cruz-Teran C.A., Tiruthani K., Mischler A., Rao B.M. Inefficient Ribosomal Skipping Enables Simultaneous Secretion and Display of Proteins in\u00a0Saccharomyces cerevisiae<\/em>, ACS Synthetic Biology 2017<\/a><\/p>\n

Canbolat, M.F., Gera, N., Tang, C., Monian, B., Rao, B.M., Pourdeyhimi, B., Khan, S.A., Preservation of Cell Viability and Protein Conformation on Immobilization within Nanofibers via Electrospinning Functionalized Yeast, ACS Applied Materials & Interfaces 2013<\/a><\/span><\/p>\n

Goli, K.K., Gera, N., Liu, X., Rao, B.M., Rojas, O.J., Genzer, J., Generation and properties of antibacterial coatings based on electrostatic attachment of silver nanoparticles to protein-coated polypropylene fibers, ACS Applied Materials & Interfaces 2013<\/a><\/span><\/p>\n

Gera, N., Hill, A.B., White, D.P., Carbonell, R.G., Rao, B.M., Design of pH Sensitive Binding Proteins from the Hyperthermophilic Sso7d Scaffold, PLoS ONE 2012<\/a><\/span><\/p>\n

Hussain, M., Lockney, D., Wang, R., Gera, N., Rao, B.M., Avidity-mediated virus separation using a hyperthermophilic affinity ligand, Biotechnology Progress 2012<\/a><\/span><\/p>\n

Menegatti, S.,Hussain, M., Naik, A., Carbonell, R.G., Rao, B.M., mRNA display selection and solid-phase synthesis of Fc-binding cyclic peptide affinity ligands, Biotechnology and Bioengineering 2012<\/a><\/span><\/p>\n

Hussain, M.*, Gera, N.*, Hill, A.B., Rao, B.M., Scaffold diversification enhances effectiveness of a super-library of hyperthermophilic proteins, ACS Synthetic Biology 2012<\/a><\/span>
\n* These authors contributed equally<\/p>\n

Gera, N., Hussain, M., Rao, B.M., Protein Selection using Yeast Cell Surface Display, Methods 2012<\/a><\/span><\/p>\n

Gera, N., Hussain, M., Wright, R.C., Rao, B.M., Highly stable binding proteins derived from the hyperthermophilic Sso7d scaffold, Journal of Molecular Biology 2011 Jun<\/a><\/span>;409(4):601-16 <\/a><\/p>\n

Cochran, J. R., Kim, Y.S., Lippow, S., Rao, B.M. and Wittrup, K. D. Improved mutants from directed evolution are biased to orthologous substitutions, Protein Engineering Design and Selection 2006 Jun<\/a><\/span>; 19(6): 245-253 <\/a><\/p>\n

Rao, B.M., Driver, I., Lauffenburger, D. A., and Wittrup, K. D. High-affinity CD25-binding IL-2 mutants potently stimulate persistent T cell growth,Biochemistry 2005 Aug<\/span>; 44(31): 10696-10701 <\/a><\/p>\n

Rao, B.M., Lauffenburger, D. A., and Wittrup, K. D. Engineering protein therapeutics within a systems-level computational context, Nature Biotechnology 2005 Feb<\/span>; 23(2): 191-194 <\/a><\/p>\n

Rao, B.M., Driver, I., Lauffenburger, D. A., and Wittrup, K. D. IL-2 variants engineered for increased IL-2R-alpha affinity exhibit increased potency arising from a cell surface ligand reservoir effect, Molecular Pharmacology 2004 Oct<\/a><\/span>; 66(4): 864-869 <\/a><\/p>\n

Rao, B.M., Girvin, A. T., Ciardelli, T., Lauffenburger, D. A., and Wittrup, K. D. Interleukin-2 mutants with increased alpha-subunit binding affinity, Protein Engineering 2003 Dec<\/a><\/span>; 16(12): 1081 1087 <\/a><\/p>\n

Stem Cell Engineering<\/h2>\n

Jabeen, M., Karakis, V., Britt, J., Miguel, A. S., & Rao, B. (2024). <\/span>A quantitative image analysis platform for assessing trophoblast differentiation.<\/span><\/i> Placenta<\/span><\/i>. <\/span>https:\/\/doi.org\/10.1016\/j.placenta.2024.07.009<\/span><\/a><\/p>\n

Karakis, V., Britt, J. W., Jabeen, M., Miguel, A. S., & Rao, B. M. (2024). <\/span>Derivation of human trophoblast stem cells from placentas at birth<\/span><\/i>. bioxRiv, <\/span>https:\/\/doi.org\/10.1101\/2024.05.01.592064<\/span><\/a>\u00a0<\/span><\/p>\n

Tiruthani. K., Sarkar, P., Rao, B.M., Trophoblast differentiation of human embryonic stem cells, Biotechnology Journal. 2013 <\/a><\/span>doi:10.1002\/biot.201200203<\/a><\/p>\n

Sarkar, P., Randall, S.M., Muddiman, D.C., Rao, B.M., Targeted proteomics of the secretory pathway reveals the secretome of mouse embryonic fibroblasts and human embryonic stem cells, Molecular & Cellular Proteomics 2012 <\/a><\/span>; Dec;11(12):1829-39 <\/a><\/p>\n

Sarkar, P., Collier, T.S., Randall, S.M., Muddiman, D.C., Rao, B.M., The subcellular proteome of undifferentiated human embryonic stem cells, Proteomics 2012 Feb<\/a><\/span> 12(3):421-30<\/a><\/p>\n

Sarkar, P., Rao, B.M., Role of Signaling Pathways and Epigenetic Factors in Lineage Determination During Human Embryonic Stem Cell Differentiation, Embryonic Stem Cells – Differentiation and Pluripotent Alternatives 2011 Oct<\/span><\/p>\n

Collier, T.S., Randall, S.M., Sarkar, P., Rao, B.M, Dean, R.A., Muddiman, D.C., Comparison of stable-isotope labeling with amino acids in cell culture and spectral counting for relative quantification of protein expression, Rapid Communications in Mass Spectrometry 2011 Sep<\/a><\/span>;25(17):2524-32 <\/a><\/p>\n

Collier, T.S., Sarkar, P., Franck, W.L., Rao, B.M., Dean, R.A., Muddiman, DC., Direct comparison of stable isotope labeling by amino acids in cell culture and spectral counting for quantitative proteomics, Analytical Chemistry 2010 Oct<\/a><\/span>;82(20):8696-702 <\/a><\/p>\n

Collier, T.S., Sarkar, P., Rao, B.M., Muddiman, D.C., Quantitative top-down proteomics of SILAC labeled human embryonic stem cells, Journal of the American Society for Mass Spectrometry 2010 Jun<\/a><\/span>;21(6):879-89 <\/a><\/p>\n

Sarkar, P., Rao, B.M., Molecular aspects of cardiac differentiation in embryonic stem cells, Critical Reviews in Biomedical Engineering 2009<\/a><\/span>;37(4-5):283-320 <\/a><\/p>\n

Varelas, X., Sakuma, R., Samavarchi-Tehrani, P., Peerani, R., Rao, B.M., Dembowy, J., Yaffe, M. B., Zandstra, P.W. and Wrana, J.L. TAZ controls Smad nucleocytoplasmic shuttling and regulates human embryonic stem-cell self-renewal, Nature Cell Biology 2008 Jul<\/a><\/span>;10(7):837-48 <\/a><\/p>\n

Peerani, R.*, Rao, B.M.*, Bauwens, C., Yin, T., Wood, G., Nagy, A., Kumacheva, E., Zandstra, P. W. Niche-mediated control of human embryonic stem cell self-renewal and differentiation, EMBO Journal 2007 Nov<\/a><\/span>; 26(22): 4744-4755<\/a>
\n* These authors contributed equally<\/p>\n

Rao, B.M. and Zandstra, P.W. Culture development for human embryonic stem cell propagation: molecular aspects and challenges, Current Opinion in Biotechnology 2005 Oct<\/a><\/span>; 16(5): 568-576 <\/a><\/p>\n

Patents<\/h2>\n

Wittrup, K. D., Rao, B.M. and Lauffenburger, D. A. Mutant Interleukin-2 Polypeptides (US Application No. 10\/894,833)<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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